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medium bulldog bio bb01 papain dissociation system worthington biochem pds polybrene millipore sigma tr 1003 g pbs  (Worthington Biochemical)


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    Worthington Biochemical medium bulldog bio bb01 papain dissociation system worthington biochem pds polybrene millipore sigma tr 1003 g pbs
    Medium Bulldog Bio Bb01 Papain Dissociation System Worthington Biochem Pds Polybrene Millipore Sigma Tr 1003 G Pbs, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 2241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbs+medium/Papain+Dissociation+System/pm41543935-252-230-237
    Average 99 stars, based on 2241 article reviews
    medium bulldog bio bb01 papain dissociation system worthington biochem pds polybrene millipore sigma tr 1003 g pbs - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Virus:

    Article Title: Human organoid tumor transplantation identifies functional glioblastoma-microenvironment communication mediated by PTPRZ1.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-SOX2 Santa Cruz SC-365823; RRID:AB_10842165 mouse anti-Nestin Millipore Sigma MAB5326; RRID:AB_2251134 chicken anti-GFP Aves GFP-1020: RRID:AB_10000240 chicken anti -GFAP Abcam AB4674: RRID:AB_304558 rabbit anti -Ki67 Abcam AB16667; RRID:AB_302459 rabbit anti-MEF2C Novus NBP1-89468;RRID:AB_11004765 rabbit anti-HOPX Proteintech 11419-1-AP;RRID:AB_10693525 mouse anti-PAX6 Abcam ab78545;RRID:AB_1566562 rabbit anti-FOXG1 Abcam ab18259;RRID:AB_732415 Rat anti -CTIP2 Abcam ab18465;RRID:AB_2064130 rabbit anti -SATB2 Abcam ab92446;RRID:AB_10563678 rabbit anti -PDGFRB Cell Signaling Tech 3169; RRID:AB_2162497 goat anti -CHI3L1 Bio-Techne AF2599; RRID:AB_2291883 Alexa Fluor 488 donkey anti-goat Invitrogen A32814; RRID:AB_2762838 Alexa Fluor 568 donkey anti-mouse Invitrogen A32773; RRID:AB_2762848 Alexa Fluor 568 donkey anti-rat Invitrogen A78946; RRID:AB_2910653 Alexa Fluor 568 donkey anti-chicken Invitrogen A11041; RRID:AB_2534098 Alexa Fluor 647 donkey anti-rabbit Invitrogen A31573; AB_2536183 Alexa Fluor 647 donkey anti-mouse Invitrogen A32787; RRID:AB_2762830 Bacterial and virus strains LV-CMV-GFP-Puro SignaGen SL100268 NEB® Stable Competent E. coli (High Efficiency) New England Biolabs C3040 Biological samples Patient Tumor Samples UCLA Neurosurgery and Brain Tumor Translational Resource See ‘‘Patient Sample Info’’ tab in Supplementary Tables Chemicals, peptides, and recombinant proteins DAPI Thermo Fisher Scientific 62248 Rock Inhibitor Y-27632 Tocris 1254 IWR-1-endo Cayman Chemical 13659 SB431542 Tocris 1614 DMEM/F12 Thermo Fisher Scientific 11330057 Pen-Strep Thermo Fisher Scientific 15140122 B27 Supplement without Vitamin A Thermo Fisher Scientific 12587010 Heparin Sigma-Aldrich H3149 Human FGF-basic Recombinant Protein Thermo Fisher Scientific PHG0263 Human EGF Recombinant Protein Thermo Fisher Scientific PHG0313 TrypLE Express Thermo Fisher Scientific 12605028 Bambanker Cell Freezing Medium Bulldog Bio BB01 Papain Dissociation System Worthington Biochem PDS Polybrene Millipore Sigma TR-1003-G PBS, pH 7.4 Thermo Fisher Scientific 10010031 DMSO Millipore Sigma D2650-100ML Trypan Blue Solution, 0.4% Thermo Fisher Scientific 15250061 Fetal Bovine Serum, Premium Thermo Fisher Scientific A5670801 (Continued on next page) Cell Reports 45, 116848, January 27, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER GMEM Thermo Fisher Scientific 11710035 mTeSR Plus STEMCELL Technologies 100–0276 ReLeSR STEMCELL Technologies 100–0483 mFreSR STEMCELL Technologies 5855 Accutase STEMCELL Technologies 07920 Knockout Serum Replacement Thermo Fisher Scientific 10828028 2-Mercaptoethanol Thermo Fisher Scientific 21985023 MEM Non-Essential Amino Acids Solution Thermo Fisher Scientific 11140050 Sodium Pyruvate Thermo Fisher Scientific 11360070 DMEM/F12 with Glutamax Thermo Fisher Scientific 10565018 N-2 Supplement Thermo Fisher Scientific 17502048 Chemically Defined Lipid Concentrate Thermo Fisher Scientific 11905031 16% Paraformaldehyde Aqueous Solution Electron Microscopy Sciences 15710 Fisher Healthcare Tissue-PlusTM O.C.T.

    Article Title: A human tumor-immune organoid model of glioblastoma.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD3 epsilon Abcam ab16669; RRID:AB_443425 CD14 Abcam ab181470; RRID:AB_2725781 CD19 Cell Signaling Technology 3574; RRID:AB_2275523 GFP Novus Bio NB100-1770; RRID:AB_10128178 Alexa Fluor 488 anti-goat (donkey host) Invitrogen A32814; RRID:AB_2762838 Alexa Fluor 647 anti-mouse (donkey host) Invitrogen A32787; RRID:AB_2762830 Alexa Fluor 647 anti-rabbit (donkey host) Invitrogen A31573; AB_2536183 CD3 Monoclonal Antibody (UCHT1), Alexa Fluor TM 700, eBioscience Thermo Fisher Scientific 56-0038-80; RRID:AB_906222 APC anti-human CD14 BioLegend 301807; RRID:AB_314189 CD19 Monoclonal Antibody (HIB19), APC-eFluor TM 780, eBioscience Thermo Fisher Scientific 47-0199-41; RRID:AB_1582231 CD45 Monoclonal Antibody (HI30), PE, eBioscience Thermo Fisher Scientific 12-0459-42; RRID:AB_1724079 Bacterial and virus strains LV-CMV-GFP-Puro SignaGen SL100268 Biological samples Patient Tumor Samples UCLA Neurosurgery and Brain Tumor Translational Resource See ‘‘Patient Sample Info’’ tab in Supplementary Tables Matched Patient Peripheral Blood Mononuclear Cells UCLA Neurosurgery and Brain Tumor Translational Resource See ‘‘Patient Sample Info’’ tab in Supplementary Tables Donor Peripheral Blood Mononuclear Cells UCLA/CFAR Virology Core Laboratory See ‘‘Patient Sample Info’’ tab in Supplementary Tables Chemicals, peptides, and recombinant proteins DAPI Thermo Fisher Scientific 62248 Rock Inhibitor Y-27632 Tocris 1254 IWR-1-endo Cayman Chemical 13659 SB431542 Tocris 1614 DMEM/F12 Thermo Fisher Scientific 11330057 GlutaMAX Thermo Fisher Scientific 35050061 Pen-Strep Thermo Fisher Scientific 15140122 B27 Supplement without Vitamin A Thermo Fisher Scientific 12587010 Heparin Sigma-Aldrich H3149 Human FGF-basic Recombinant Protein Thermo Fisher Scientific PHG0263 Human EGF Recombinant Protein Thermo Fisher Scientific PHG0313 TrypLE Express Thermo Fisher Scientific 12605028 Bambanker Cell Freezing Medium Bulldog Bio BB01 Papain Dissociation System Worthington Biochem PDS Polybrene Millipore Sigma TR-1003-G PBS, pH 7.4 Thermo Fisher Scientific 10010031 Ficoll-Paque Cytiva 17544202 Trypan Blue Solution, 0.4% Thermo Fisher Scientific 15250061 Fetal Bovine Serum, Premium Thermo Fisher Scientific A5670801 DMSO Millipore Sigma D2650-100ML (Continued on next page) 14 Cell Reports 45, 116790, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER mTeSR Plus STEMCELL Technologies 100–0276 Matrigel Corning 354230 Primocin InvivoGen ant-pm-1 ReLeSR STEMCELL Technologies 100–0483 mFreSR STEMCELL Technologies 5855 Accutase STEMCELL Technologies 07920 GMEM Thermo Fisher Scientific 11710035 Knockout Serum Replacement Thermo Fisher Scientific 10828028 2-Mercaptoethanol Thermo Fisher Scientific 21985023 MEM Non-Essential Amino Acids Solution Thermo Fisher Scientific 11140050 Sodium Pyruvate Thermo Fisher Scientific 11360070 DMEM/F12 with Glutamax Thermo Fisher Scientific 10565018 N-2 Supplement Thermo Fisher Scientific 17502048 Chemically Defined Lipid Concentrate Thermo Fisher Scientific 11905031 RPMI 1640 Medium Thermo Fisher Scientific 11875093 16% Paraformaldehyde Aqueous Solution Electron Microscopy Sciences 15710 Fisher Healthcare Tissue-Plus TM O.C.T.

    Recombinant:

    Article Title: Human organoid tumor transplantation identifies functional glioblastoma-microenvironment communication mediated by PTPRZ1.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-SOX2 Santa Cruz SC-365823; RRID:AB_10842165 mouse anti-Nestin Millipore Sigma MAB5326; RRID:AB_2251134 chicken anti-GFP Aves GFP-1020: RRID:AB_10000240 chicken anti -GFAP Abcam AB4674: RRID:AB_304558 rabbit anti -Ki67 Abcam AB16667; RRID:AB_302459 rabbit anti-MEF2C Novus NBP1-89468;RRID:AB_11004765 rabbit anti-HOPX Proteintech 11419-1-AP;RRID:AB_10693525 mouse anti-PAX6 Abcam ab78545;RRID:AB_1566562 rabbit anti-FOXG1 Abcam ab18259;RRID:AB_732415 Rat anti -CTIP2 Abcam ab18465;RRID:AB_2064130 rabbit anti -SATB2 Abcam ab92446;RRID:AB_10563678 rabbit anti -PDGFRB Cell Signaling Tech 3169; RRID:AB_2162497 goat anti -CHI3L1 Bio-Techne AF2599; RRID:AB_2291883 Alexa Fluor 488 donkey anti-goat Invitrogen A32814; RRID:AB_2762838 Alexa Fluor 568 donkey anti-mouse Invitrogen A32773; RRID:AB_2762848 Alexa Fluor 568 donkey anti-rat Invitrogen A78946; RRID:AB_2910653 Alexa Fluor 568 donkey anti-chicken Invitrogen A11041; RRID:AB_2534098 Alexa Fluor 647 donkey anti-rabbit Invitrogen A31573; AB_2536183 Alexa Fluor 647 donkey anti-mouse Invitrogen A32787; RRID:AB_2762830 Bacterial and virus strains LV-CMV-GFP-Puro SignaGen SL100268 NEB® Stable Competent E. coli (High Efficiency) New England Biolabs C3040 Biological samples Patient Tumor Samples UCLA Neurosurgery and Brain Tumor Translational Resource See ‘‘Patient Sample Info’’ tab in Supplementary Tables Chemicals, peptides, and recombinant proteins DAPI Thermo Fisher Scientific 62248 Rock Inhibitor Y-27632 Tocris 1254 IWR-1-endo Cayman Chemical 13659 SB431542 Tocris 1614 DMEM/F12 Thermo Fisher Scientific 11330057 Pen-Strep Thermo Fisher Scientific 15140122 B27 Supplement without Vitamin A Thermo Fisher Scientific 12587010 Heparin Sigma-Aldrich H3149 Human FGF-basic Recombinant Protein Thermo Fisher Scientific PHG0263 Human EGF Recombinant Protein Thermo Fisher Scientific PHG0313 TrypLE Express Thermo Fisher Scientific 12605028 Bambanker Cell Freezing Medium Bulldog Bio BB01 Papain Dissociation System Worthington Biochem PDS Polybrene Millipore Sigma TR-1003-G PBS, pH 7.4 Thermo Fisher Scientific 10010031 DMSO Millipore Sigma D2650-100ML Trypan Blue Solution, 0.4% Thermo Fisher Scientific 15250061 Fetal Bovine Serum, Premium Thermo Fisher Scientific A5670801 (Continued on next page) Cell Reports 45, 116848, January 27, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER GMEM Thermo Fisher Scientific 11710035 mTeSR Plus STEMCELL Technologies 100–0276 ReLeSR STEMCELL Technologies 100–0483 mFreSR STEMCELL Technologies 5855 Accutase STEMCELL Technologies 07920 Knockout Serum Replacement Thermo Fisher Scientific 10828028 2-Mercaptoethanol Thermo Fisher Scientific 21985023 MEM Non-Essential Amino Acids Solution Thermo Fisher Scientific 11140050 Sodium Pyruvate Thermo Fisher Scientific 11360070 DMEM/F12 with Glutamax Thermo Fisher Scientific 10565018 N-2 Supplement Thermo Fisher Scientific 17502048 Chemically Defined Lipid Concentrate Thermo Fisher Scientific 11905031 16% Paraformaldehyde Aqueous Solution Electron Microscopy Sciences 15710 Fisher Healthcare Tissue-PlusTM O.C.T.

    Article Title: A human tumor-immune organoid model of glioblastoma.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD3 epsilon Abcam ab16669; RRID:AB_443425 CD14 Abcam ab181470; RRID:AB_2725781 CD19 Cell Signaling Technology 3574; RRID:AB_2275523 GFP Novus Bio NB100-1770; RRID:AB_10128178 Alexa Fluor 488 anti-goat (donkey host) Invitrogen A32814; RRID:AB_2762838 Alexa Fluor 647 anti-mouse (donkey host) Invitrogen A32787; RRID:AB_2762830 Alexa Fluor 647 anti-rabbit (donkey host) Invitrogen A31573; AB_2536183 CD3 Monoclonal Antibody (UCHT1), Alexa Fluor TM 700, eBioscience Thermo Fisher Scientific 56-0038-80; RRID:AB_906222 APC anti-human CD14 BioLegend 301807; RRID:AB_314189 CD19 Monoclonal Antibody (HIB19), APC-eFluor TM 780, eBioscience Thermo Fisher Scientific 47-0199-41; RRID:AB_1582231 CD45 Monoclonal Antibody (HI30), PE, eBioscience Thermo Fisher Scientific 12-0459-42; RRID:AB_1724079 Bacterial and virus strains LV-CMV-GFP-Puro SignaGen SL100268 Biological samples Patient Tumor Samples UCLA Neurosurgery and Brain Tumor Translational Resource See ‘‘Patient Sample Info’’ tab in Supplementary Tables Matched Patient Peripheral Blood Mononuclear Cells UCLA Neurosurgery and Brain Tumor Translational Resource See ‘‘Patient Sample Info’’ tab in Supplementary Tables Donor Peripheral Blood Mononuclear Cells UCLA/CFAR Virology Core Laboratory See ‘‘Patient Sample Info’’ tab in Supplementary Tables Chemicals, peptides, and recombinant proteins DAPI Thermo Fisher Scientific 62248 Rock Inhibitor Y-27632 Tocris 1254 IWR-1-endo Cayman Chemical 13659 SB431542 Tocris 1614 DMEM/F12 Thermo Fisher Scientific 11330057 GlutaMAX Thermo Fisher Scientific 35050061 Pen-Strep Thermo Fisher Scientific 15140122 B27 Supplement without Vitamin A Thermo Fisher Scientific 12587010 Heparin Sigma-Aldrich H3149 Human FGF-basic Recombinant Protein Thermo Fisher Scientific PHG0263 Human EGF Recombinant Protein Thermo Fisher Scientific PHG0313 TrypLE Express Thermo Fisher Scientific 12605028 Bambanker Cell Freezing Medium Bulldog Bio BB01 Papain Dissociation System Worthington Biochem PDS Polybrene Millipore Sigma TR-1003-G PBS, pH 7.4 Thermo Fisher Scientific 10010031 Ficoll-Paque Cytiva 17544202 Trypan Blue Solution, 0.4% Thermo Fisher Scientific 15250061 Fetal Bovine Serum, Premium Thermo Fisher Scientific A5670801 DMSO Millipore Sigma D2650-100ML (Continued on next page) 14 Cell Reports 45, 116790, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER mTeSR Plus STEMCELL Technologies 100–0276 Matrigel Corning 354230 Primocin InvivoGen ant-pm-1 ReLeSR STEMCELL Technologies 100–0483 mFreSR STEMCELL Technologies 5855 Accutase STEMCELL Technologies 07920 GMEM Thermo Fisher Scientific 11710035 Knockout Serum Replacement Thermo Fisher Scientific 10828028 2-Mercaptoethanol Thermo Fisher Scientific 21985023 MEM Non-Essential Amino Acids Solution Thermo Fisher Scientific 11140050 Sodium Pyruvate Thermo Fisher Scientific 11360070 DMEM/F12 with Glutamax Thermo Fisher Scientific 10565018 N-2 Supplement Thermo Fisher Scientific 17502048 Chemically Defined Lipid Concentrate Thermo Fisher Scientific 11905031 RPMI 1640 Medium Thermo Fisher Scientific 11875093 16% Paraformaldehyde Aqueous Solution Electron Microscopy Sciences 15710 Fisher Healthcare Tissue-Plus TM O.C.T.

    Incubation:

    Article Title: Molecularly Characterised Xenograft Tumour Mouse Models: Valuable Tools for Evaluation of New Therapeutic Strategies for Secondary Liver Cancers
    Article Snippet: .. Human tumour samples with an average size of 1 cm 3 were cut into 2 × 2 mm pieces in the presence of digestion medium (PBS/2 mg/mL collagenase III; 37°C; Worthington Biochemical Corporation, NJ, USA), transferred into 15 mL tubes (Sarstedt, Wiener Neustadt, Austria) and further incubated for 1 hour at 37°C with continuous shaking at 320 cycles per minute (Thermoshaker HTMR 132; Haep Labor Consult, Bovenden, Germany). ..



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    Image Search Results


    Analysis of 3D Raman image stacks of infected BGM cells at time points 18, 36, 48 and 54 h post infection (p.i.) . A , bright field overview images of BGM cells infected with C . abortus at different hours p.i. The red square highlights the region (10 μm × 10 μm) that was further analyzed by Raman spectroscopic imaging. This scale bar represent 10 μm. B , false color Raman images of the three z-planes measured in the region highlighted in panel A . Blurring filter and 3D perspective were applied for better visualization. The color code is the same throughout the panels B – D : white - PBS/water, orange - DNA/nucleus, dark red - RB of C . abortus , mid blue - EB of C . abortus , light green - cytoplasm of the host cell. The false color image is a superposition of the relative abundance of each endmember in each pixel. The scale bars represent 1 μm. The distance between z-layers is 1 μm. C , gray-scale maps showing abundance distributions of C . abortus endmembers in the middle layer shown in panel B . Color gradients were corrected to make lower values more visible. D , endmember spectra corresponding to the abundances shown in panels B and C . For C . abortus , the spectra are overlaid with the corresponding endmember spectrum of isolated bacteria from I for comparison. The spectra of isolated C . abortus are upscaled to simplify visual comparison. Original values are provided in I . , and provide the same information for all eight Raman images without additional effects. BGM, buffalo green monkey; EB, elementary body; RB, reticulate body.

    Journal: The Journal of Biological Chemistry

    Article Title: Insights into chlamydial infection at the sub-cellular level using label-free Raman spectroscopy in comparison to electron microscopy

    doi: 10.1016/j.jbc.2025.110940

    Figure Lengend Snippet: Analysis of 3D Raman image stacks of infected BGM cells at time points 18, 36, 48 and 54 h post infection (p.i.) . A , bright field overview images of BGM cells infected with C . abortus at different hours p.i. The red square highlights the region (10 μm × 10 μm) that was further analyzed by Raman spectroscopic imaging. This scale bar represent 10 μm. B , false color Raman images of the three z-planes measured in the region highlighted in panel A . Blurring filter and 3D perspective were applied for better visualization. The color code is the same throughout the panels B – D : white - PBS/water, orange - DNA/nucleus, dark red - RB of C . abortus , mid blue - EB of C . abortus , light green - cytoplasm of the host cell. The false color image is a superposition of the relative abundance of each endmember in each pixel. The scale bars represent 1 μm. The distance between z-layers is 1 μm. C , gray-scale maps showing abundance distributions of C . abortus endmembers in the middle layer shown in panel B . Color gradients were corrected to make lower values more visible. D , endmember spectra corresponding to the abundances shown in panels B and C . For C . abortus , the spectra are overlaid with the corresponding endmember spectrum of isolated bacteria from I for comparison. The spectra of isolated C . abortus are upscaled to simplify visual comparison. Original values are provided in I . , and provide the same information for all eight Raman images without additional effects. BGM, buffalo green monkey; EB, elementary body; RB, reticulate body.

    Article Snippet: Intact, infected BGM cells were analyzed directly in aqueous medium (PBS) with a 60× water immersion objective (NA 1, Nikon).

    Techniques: Infection, Imaging, Isolation, Bacteria, Comparison